DILUTION FACTOR

I was really get confused what DILUTING is about...
Some problems when they come to calculate & determine analytically related to dilution factor, I'm getting pissed off! hoho
Sorry, I suddenly become emo... haha
Okay, now I just found this article that explain about it...
Let's check it out~~~

Aliquot: a measured sub-volume of original sample.

Diluent: material with which the sample is diluted

Dilution factor (DF): ratio of final volume/aliquot volume (final volume = aliquot + diluent)

Concentration factor (CF): ratio of aliquot volume divided by the final volume (inverse of the dilution factor)
 

To calculate a dilution factor:

Remember that the dilution factor is the final volume/aliquot volume.

EXAMPLE: What is the dilution factor if you add 0.1 mL aliquot of a specimen to 9.9 mL of diluent?

1.  The final volume is equal the the aliquot volume plus the diluent volume:  0.1 mL + 9.9 mL = 10 mL
2. The dilution factor is equal to the final volume divided by the aliquot volume: 10 mL/0.1 mL = 1:100 dilution (10 ²)

The Concentration Factor for this problem = aliquot volume/final volume = 0.1/(0.1 + 9.9) = 0.01 or 10 ^-2 concentration
 

To prepare a desired volume of solution of a given dilution:

1. Calculate the volume of the aliquot: it is equal either to

• the final volume/dilution factor

or

• the concentration factor x final volume

2. Calculate the volume of the diluent: which is equal to (the final volume - aliquot volume)

3. Measure out the correct volume of diluent, add the correct volume of aliquot to it, mix.

EXAMPLE:  How would you prepare 20 mL of a 1:50 dilution?

1. Determine required aliquot by dividing final volume by dilution factor:  20 mL/50 = 0.4 mL sample
2. Subtract the aliquot volume from the final volume:  20 mL - 0.4 mL = 19.6 mL diluent
3. Measure out 19.4 mL diluent, add 0.4 mL sample to it, mix thoroughly